Charlotte the stingray, pregnant fish despite no male companion, has died

FILE - A Bluespotted stingray swims in the Aquarium of the Pacific complex in Long Beach, California, on Nov. 8, 2006. (Photo credit: GABRIEL BOUYS/AFP via Getty Images)

Charlotte, a stingray that got pregnant at a North Carolina aquarium despite not having shared a tank with a male of her species for many years, has died.

The Aquarium and Shark Lab in Hendersonville on Sunday shared that Charlotte died after getting a rare reproductive disease without going into further detail.

"We are sad to announce, after continuing treatment with her medical care team and specialist, our ray Charlotte passed away today," the aquarium wrote. 

It also thanked fans for their "continued love and support while we navigate this great loss."

The aquarium, which is in the Blue Ridge Mountains, announced in February that Charlotte had gotten pregnant despite not having shared a tank with a male stingray for at least eight years. 

The pregnancy made headlines around the world and provided fodder for late-night television host Jimmy Kimmel and "Saturday Night Live."

In May, the aquarium revealed that Charlotte was suffering from a rare reproductive disease, and that it was trying to find out more about the malady.

"There have been studies done on southern rays, but we have not found any yet done on round rays," it wrote.

The aquarium announced in early June that Charlotte was no longer pregnant. The development led the facility to temporarily close to the public on June 1. 

In its post on Sunday, the aquarium said it would remain closed for the time being and that staff would continue to feed and care for the other animals.

The small aquarium is run by Ramer’s educational nonprofit, Team ECCO, which encourages local schoolchildren and others to take an interest in science.

How does a stingray get pregnant without a mate?

The pregnancy was thought to be the result of a type of asexual reproduction called parthenogenesis, in which offspring develop from unfertilized eggs, meaning there is no genetic contribution by a male. 

The mostly-rare phenomenon can occur in some insects, fish, amphibians, birds and reptiles, but not in mammals. 

Documented examples have included California condors, Komodo dragons and yellow-bellied water snakes.

This story was reported from Cincinnati. The Associated Press contributed.